| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 771 | Frankia casuarinae | TGAAACCGCTGCTCGTACT | AGGTACCGGTTCAGGCTGA | 59.34 | 60.23 | 253 | 62 |
100.00%
|
100.00%
|
| Primer 772 | Frankia casuarinae | GTGAAACCGCTGCTCGTAC | AGGTACCGGTTCAGGCTGA | 59.22 | 60.23 | 254 | 62 |
100.00%
|
100.00%
|
| Primer 773 | Frankia casuarinae | AGATCGGTTCCGGTGGCTA | TGATCAGTGCCGTGACGTC | 60.38 | 60.08 | 153 | 62 |
100.00%
|
100.00%
|
| Primer 774 | Frankia casuarinae | TCCACGTCAGCATCCGGAT | AGTCGCTGATCCGCTTCCT | 61.06 | 61.06 | 180 | 62 |
100.00%
|
100.00%
|
| Primer 775 | Frankia casuarinae | ATCCACGTCAGCATCCGGA | AGTCGCTGATCCGCTTCCT | 61.06 | 61.06 | 181 | 62 |
100.00%
|
100.00%
|
| Primer 776 | Frankia casuarinae | GGCAGAACGTCCCGATTCA | TCGACGAGGAAACACTGGC | 60.08 | 60.01 | 181 | 62 |
100.00%
|
100.00%
|
| Primer 777 | Frankia casuarinae | TGCGCAACAAGGCTGTGA | TGGCGTGAATGACACCCT | 60.52 | 58.84 | 153 | 62 |
100.00%
|
100.00%
|
| Primer 778 | Frankia casuarinae | ATGATCGCGTCGTTGGC | CTGCAGGATCTCGAAGACGT | 58.17 | 59.55 | 225 | 62 |
100.00%
|
100.00%
|
| Primer 779 | Frankia casuarinae | ATCGCGACCAGGTGATCCT | AGACGCACAGCAGCCATT | 60.76 | 59.97 | 153 | 62 |
100.00%
|
100.00%
|
| Primer 780 | Frankia casuarinae | GTTCGAGGTGACCGAGCAT | ACTTCGACGAACAGCCCT | 59.78 | 58.54 | 291 | 62 |
100.00%
|
100.00%
|